23rd NZ Fungal Foray report

THE 23rd NZ FUNGAL FORAY, WAIKANAE, May 2009

Petra White

Introduction

The 23rd New Zealand Fungal Foray was held conjointly with the Australasian Mycological Society Conference 10–16 May 2009 at Waikanae on the Kapiti Coast, Wellington. Accommodation was at El Rancho Christian Holiday Camp and 33 people attended the foray. Visitors came from USA, Australia, Tasmania, Japan, England and Sweden.

Prior to European settlement the Kapiti coast consisted of bands of vegetation zones running north-south along the coastline. Adjacent to the coast there was a wide band of duneland colonised by grassland and shrublands. Further inland there was a narrow band of kohekohe (Dysoxylum spectabile)-karaka (Corynocarpus laevigatus)-ngaio (Myoporum laetum) coastal forest. Beyond this, on the alluvial flats right up to the edges of SH1, was a band of swamp forest containing kahikatea (Dacrycarpus dacrydioides), pukatea (Laurelia novae-zelandiae), maire tawaki (Syzygium maire) and ti kouka (Cordyline australis) (Gabites 1993).

On the inland side of what is now SH1, tawa (Beilschmiedia tawa), rewarewa (Knightia excelsa), hinau (Elaeocarpus dentatus), nikau (Rhopalostylis sapida), mapou (Myrsine australis), porokaiwhiri (Hedycarya arborea) and mahoe (Melicytus ramiflorus) entered the canopy. Beyond this again was a band of podocarp/broadleaf forest that extended right down the middle of the Wellington peninsula, predominantly tawa forest with emergent rimu (Dacrydium cupressinum) and northern rata (Metrosideros robusta). From around 400m kamahi (Weinmannia racemosa) and hinau replaced tawa in the canopy and red beech (Nothofagus fusca) and silver beech (N. menziesii) were prominent. Above 550 m northern rata, rimu and hinau were replaced by miro (Prumnopitys ferruginea) and Hall’s totara (Podocarpus cunninghamii). Above 800 m there was a band of silver beech (Gabites, 1993).

The European arrival saw rapid destruction of these vast forests for pastoral farming. Very little of these original forests now remain in the Wellington region, and they are mostly found in the hill country, predominantly beech. Much of the surviving original forest around Waikanae still stands because of the efforts of William (“Willie”) Hughes Field, who once owned 3100 acres (1254.5 ha) of coastal plain in the area and was one of the founders, in 1903, of the Scenery Preservation Society, a small group of powerful men who advocated wherever possible the preservation of New Zealand’s forest heritage throughout the country (White 2001).

This foray added 695 fungal records (representing 362 taxa) to the FUNNZ database, and 389 collections to the New Zealand Fungal and Plant Disease Herbarium (PDD). New records for New Zealand included Leucopaxillus lilacinus, Omphalina pyxidata, and Sirobasidium rubrofuscum. There were 65 records of 48 taxa listed as ‘Data deficient’ and one record of Chalciporus aurantiaca, which is currently listed as ‘Nationally Critical’, but probably needs reassessment. The sites with most collections were Rimutaka Forest Park (164), Lake Papaitonga (79) and Nga Manu Nature Reserve (75).

Monday 11 May, keeping it close

We decided to visit nearby reserves for the first day of foraying. After breakfast we traveled south on SH1 to Nikau Reserve. The reserve is dominated by kohekohe and nikau with tawa. Common fungi on wood were the introduced orange poreconch (Favolaschia calocera), sunset leatherbracket (Stereum ostrea) and artist’s porebracket (Ganoderma cf. applanatum). By the edge of the track we found a group of earthstars (Geastrum saccatum).

We walked uphill to a grassy hilltop from which we could look down over Paraparaumu to Kapiti Island in the distance. The vegetation changed and tree daisy (Olearia sp.) became frequent. On the way down just off the track I found a colony of a cup fungus (Cookeina colensoi) growing on wood.

After lunch we went to nearby Paraparaumu Scenic Reserve, a 256 ha reserve accessed from SH1 via a covenant area. This reserve is dominated by kohekohe, tawa and mahoe. On the valley floor we found a huge old kohekohe and a tangle of supplejack (Ripogonum scandens) amongst wheki (Dicksonia squarrosa). There was not much in the way of fungi to be found, the most common being orange poreconch again. Growing from soil I found a couple of scarlet pouch fungi (Leratiomyces erythrocephalus).

Next we went back north to Waikanae and visited the 330 ha Hemi Matenga Memorial Park Scenic Reserve. Here tall kohekohe were festooned with a jumble of supplejack. Once again the orange poreconch was everywhere, but very little else. The native fern poreconch (Favolaschia cyatheae) was found growing on Cyathea debris and wood-ear jelly (Auricularia cornea) was very common growing on wood.

Tuesday 12 May, heading north

Our first stop this day was to Nga Manu Nature Reserve. This is 15 ha of lowland swamp forest dominated by 400-year-old kahikatea. The reserve boasts 700 different native plant species, many of which are on the threatened list, including native grasses and ferns. There is a captive-breeding programme under way as well as revegetation and weed control. Mistletoe has been introduced into the reserve, the most successful species being Ileostylus micranthus. The idea of building a predator-proof fence around the reserve was rejected as it would involve the removal of too much native forest to make way for it.

A highlight of the visit was the finding of a club fungus, Macrotyphula defibulata), growing on the decaying stems of NZ flax (Phormium tenax) at the edge of the boardwalk. Another species found on the flax, this time the leaves, was Melanotus vorax, currently only known from two other collections. On the way out, growing on the road reserve we found a dozen or so witch-hat waxgills (Hygrocybe conica) peeping through the grass. In the same area were several puffballs (Bovista sp.), some opened out to display the spore mass ready for dispersal.

After lunch we headed north to Waiopehu Scenic Reserve in Levin. The vegetation in this 9 ha reserve is tawa forest and turned out to be quite fruitful in terms of fungi. There were lots of fallen trees creating habitat for various wood-dwelling fungi. A jelly fungus (Tremella sp.) was quite common, as was wood-ear jelly and ivory conch (Conchomyces bursiformis). The introduced orange poreconch was present but not abundant. On a rotting log I found a group of three wineglass leatherbracket (Podoscypha petalodes subsp. floriformis).

That day at Akataroa Saddle there were two collections made of Pholiota malicola, which had first been found at Westport in 2006, the first New Zealand record. These new collections were the first North Island records.

Wednesday 13 May, 8th NZ Fungal Foray Mycology Colloquium and 2009 Australasian Mycological Society Conference

Peter Johnston, Landcare Research, started the day with a talk on accumulating and managing the DNA sequence data for New Zealand fungi. In-house projects at Landcare Research are generating a lot of sequence data and users want access to that data. Information is delivered through the NZ Fungi database but it would be a much more powerful resource if we could add DNA information to the database. Such data for instance could benefit the Department of Conservation (DoC) data deficient fungi.

Professor Brandon Matheny, University of Tennessee, then spoke of the evolution of Australasian Inocybaceae. In the Paleogene, 65-24 million years ago, there was a dramatic climate change, a cooling down and eventual isolation of Australasia. Egon Horak described 17 species of Inocybe, but this genus deserves more work, as there are a lot more species than we think.

Kentaro Hosaka, from Japan’s National Museum of Nature and Science, described some preliminary results from multigene analysis of truffle-like fungi (Hysterangiales) and earthstars (Geastrales), two of the four major groups within Phallomycetidae, to understand the comparative biogeography of closely related but ecologically distinct groups. Earthstars grow above ground, are saprotrophic and spores are wind-dispersed, whereas the truffle-like fungi grow below ground, are ectomycorrhizal and small animals disperse the spores. The other two groups in Phallomycetidae are stinkhorns (Phellales) and coral and club fungi (Gomphales). Comparative biogeography of these four closely related groups will provide exciting incite into the fungal biogeography, still in a developing stage compared to plant and animal studies.

Next was Judy Gardner from Scion, who described her work on stream baiting for Phytophthora spp. as a surveillance toll in New Zealand. The genus Phytophthora is an Oomycete, which was previously included in the fungal kingdom but is now in kingdom Chromista. Phytophthora spp., commonly known as water moulds, produce motile zoospores and require water for dispersal. They are responsible for some of the most destructive plant diseases known to man. Examples include European potato famine of the 19th century (caused by P. infestans and P. cinnamomi), sudden oak death (P. ramorum) in western USA, and kauri dieback in New Zealand (P. taxon Agathis). To enable the evaluation for conditions in New Zealand and investigate the presence of Phytophthora spp. a series of traps were set up in six streams on the Volcanic Plateau. During a 12-month period over 300 cultures were collected for further evaluation.

Katrin Walbert, also from Scion, described her work on ectomycorrhizal fungi (ECM) in plantation forests of New Zealand. ECMs increase root spread by more than 40 times so that there is more uptake of nutrients by plants. The presence of these fungi can reduce the need for fertiliser. An examination of a nursery and four Pinus radiata stands of varying age in Kaingaroa Forest revealed 18 ECM species growing above ground and 19 ECM species growing below ground. The overall species richness and diversity was found to be low compared to similar forests in the Northern Hemisphere but similar to other exotic plantations in the Southern Hemisphere. ECM species identified in the nursery survived the first year of outplanting but were found to be completely replaced by forest ECMs after seven years, with the first non-nursery species appearing after six months. An earthball, Rhizopogon rubescens, was the most persistent and abundant ECM species.

Benjamin Myles, University of Otago, then spoke about his molecular phylogenetic work on the genus Menegazzia, which he described as New Zealand’s “holiest” (most perforated) lichen. Menegazzia is a genus of around 70 lichenised ascomycete species found throughout Australasia and South America. They are characterised by the easily noticeable perforations found throughout their upper thallus (apart from M. eperforata, which has none). They belong in the family Parmeliaceae, containing over 2000 species. There are 20 species in New Zealand, 12 being endemic. With Benjamin present our display tables were a little more diverse than usual with lichens appearing among the fungi.

Next Michael Lucas, University of Otago, presented work he and David Orlovich were doing on the genetic diversity of Cortinarius rotundisporus in Australia and New Zealand. Cortinarius is the most diverse ECM genus and very common in these two countries. C. rotundisporus has a blue-green pileus with a variable yellow central region and brown spores. It associates with Eucalyptus, Casuarina, Leptospermum and Kunzea spp. A study of genetic variation in C. rotundisporus (Sawyer et. al. 1999) found three phylogenetically distinct internal transcribed spacer (ITS) types (RFLP Types I, II and III), but the relationships of these ITS types to other described species was not investigated. Their preliminary results revealed 2 mostly Australian clades and 2 exclusively New Zealand ones in Type I, whereas Type II was related to C. tessiae and Type III was not related to the other two but rather to Cortinarius subgen. Dermacybe.

The next talk, entitled “The Time to Foray”, was by David Ratkowsky, University of Tasmania. He told us that the Aborigines had a different idea of seasonology. They knew a lot about the edibility of fungi but did not necessarily used fungi as an indicator of seasons. In Tasmania they had three seasons – wegtellanyta (December-April), tunna (May-August) and pawenya peena (September-November). Lists of macrofungi species from three separate studies in the Warra long-term ecological research site in southern Tasmania, where fortnightly visits were made over a 12-month period, show that the majority of species recorded were during the tunna season. David’s conclusion was that tunna is the best season to foray for fungi, particularly May-June.

After lunch Rytas Vigalys, Duke University, talked about the work he did probing fungal diversity using DNA sequence libraries, which revealed a diverse community of eukaryotic microorganisms in soils from southeastern USA Peidmont forests. These communities are dominated by fungi, but also include protistan, chorophyte and metazoan lineages. Using phylogenetic analysis and ITS sequences from basidiome surveys and environmental sources, identification to species level was possible for many common Agaricomycetes such as Russula, Suillus, Mycena and Gymnopus. This sequence data is being used to study how fungal communities respond to environmental perturbation. Examples include study of community shifts in response to CO2 enrichment and community response to long-term land use histories with different recover histories.

Then Teresa Lebel from the Royal Botanic Gardens, Melbourne spoke on the demise of the sequestrate genus Endoptychum, truffle-like forms of Agaricales where the spore mass is enclosed. This genus is now known to be an assemblage of species that are not closely related. The type E. agaricoides was found to be more closely related to the genus Chlorophyllum and is now listed as C. agaricoides. Endoptychum depressum was found to be more closely related to Agaricus and is now known as A. inapertus. Finally Chlorophyllum was conserved against the name Endoptychum, which left several European and all Australian  species of Endoptychum in nomenclature limbo. Examination of hundreds of collections revealed the presence of eight Australian species, which analysis of DNA sequences suggests has affinities with several lineages of Agaricaceae, including Macrolepiota, Agaricus and Chlorophyllum.

Dee Carter, University of Sydney, gave a talk on the basidiomycete yeast genus Cryptococcus, a true fungal pathogen acquired from the environment and established first in the lungs. The fungus is not returned to the environment unless the host dies. It is capable of causing life-threatening diseases in mammals, including humans. Cryptococcus neoformas, found in high numbers in pigeon guano, causes a severe form of meningitis and meningo-encephalitis in people with AIDS. Cryptococcus gattii affects otherwise healthy people, is more restricted in distribution and is associated with decaying wood.

The next talk, from Bevan Weir, Landcare Research, concerned the ascomycete genus Colletotrichum (teleomorph: Glomerella), a plant pathogen affecting cereals, grasses, legumes, vegetables and fruit. Typical disease symptoms are characterised by sunken necrotic lesions with orange conidia. Colletotrichum gloeosporioides causes quince (Cydonia oblonga) and apple (Malus domestica) bitter rot, anthracnose on many fruit and vegetable species such as mango (Mangifera indica) or cultivated plants like St. John’s wort (Hypericum perforatum), and rubber leaf spot on Hevea brasiliensis. Multiple gene sequences from a global collection of vouchered specimens were used to identify genetically distinct groups within C. gloeosporioides. He concluded that multiple gene sequences, appropriately analysed, can be used in conjunction with other characteristics to define biologically meaningful species and subspecies.

Pam Catcheside, Flinders University, then told us how she and her husband David had 10 years previously started to document the fungi of South Australia, including Kangaroo Island. A number of “fungal hotspots” (high species diversity, high numbers of fruiting bodies, fungi of conservation importance) have been identified from surveys in parks in seven regions of the State since 1997. One hotspot, Stringybark Walking Trail in Deep Creek Conservation Park, has remnant vegetation of Australian oak (Eucalyptus obliqua) and an understory of tufted grass tree (Xanthorrhoea semiplana subsp. semiplana). The number and diversity of fungal species in this small area exceeds that at all other locations surveyed, with the exception of the much larger and ecologically more diverse Flinders Chase National Park on Kangaroo Island. Australia lags behind other countries in assessing the conservation status of fungi. A recent study (Grgurinovic and Simpson 2001) assessed 443 fungi for rarity but this was based only on the collections of J. B. Cleland. The Catchesides want to get fungi on the threatened species lists for South Australia. One example is Mucronella pendula, which is only known from one rotting log.

Genevieve Gates, University of Tasmania, followed with a talk entitled “A Bunch of No-Good Rotters.” Sustainable forestry in Tasmania aims to retain all elements of a natural forest cycle in its management plan. In the wet Australian oak forests of southern Tasmania, wildfire at different intervals has produced a mosaic of multi-aged stands with a successional climax of temperate rainforest after 400 years in the absence of fire. There are several heart rot polypores (Fomes hemitephrus, Australoporus tasmanicus, Phellinus wahlbergii) found in these forests that appear to be either host-specific or confined to fruiting on large diameter eucalypts. Such trees are found in the older forests (>250 years) or as legacies of wildfire disturbance in the younger stands. The logging of old growth forests, the silviculture treatment of clearfell, burn and sow, and current rotation lengths of 80-100 years will see the loss of large diameter trees and the consequent risk of local disappearance of these polypores.

The last speaker for the day was Peter Johnston, Landcare Research. He was getting together a revised list of threatened fungi that he hoped to have compiled by the end of the year and then published. The initial list of 2002 only had macrofungi. Lichens were not included in the threatened species list. The previous year the DoC  had asked for an update but nothing eventuated except one submission from Pat Leonard.

Thursday 14 May, Rimutaka Forest Park

This day we drove to Rimutaka Forest Park, where we were greeted by a forest of hard beech (Nothofagus truncata). The introduced scarlet flycap (Amanita muscaria) was common by the edge of the track, some with quite large fruiting bodies. Other common species on the forest floor were the common basket stinkhorn (Ileodictyon cibarium) and crimson helmet (Mycena ura). Common fungi on wood included Fomes hemitephrus and a Laetiporus.

A new species of Russula was found. We found Cortinarius ophryx, a rare species that had only been recorded several times before in the South Island, and I found a seldom-collected species, Hygrophorus umbriceps.

Friday 15 May, Otaki Forks

The last day of foraying was a bit of a disaster day for us. We traveled north again, to Otaki Forks, an area of regenerating lowland to low montane scrub and podocarp/broadleaved forest. It began to rain as we started our foray and got heavier and heavier. Not much was found. Fungal finds of note included Cortinarius rotundisporus, Galerina patagonica, Insiticia roseoflava, Leratiomyces erythrocephalus, Podoserpula petaloides subsp. floriformis, and several fruiting bodies of Crepidotus praecipuus). We cut the foray short and made our way back to the vehicle.

References

Gabites, Isabel. 1993: Wellington’s Living Cloak – A Guide to the Natural Plant Communities, Victoria University Press.

Grgurinovic, C.A; Simpson, J.A. 2001: Conservation Status of the known Agaricales, Boletales, Cantharellales, Lycoperdales, Phallales and Russulales of South Australia. Fungal Diversity: 97-127.

Sawyer, N.A.; Chambers, S.M; Cairney, J.W.G. 1999: Molecular investigation of genet distribution and genetic variation of Cortinarius rotundisporus in eastern Australian sclerophyll forests. New Phytologist 142:561–568.

White, P. 2001: Field Reserve Management Plan, Royal Forest and Bird Protection Society, Wellington.

Web Sites

http://www.funnz.org.nz

http://nzfungi2.landcareresearch.co.nz/

http://www.hiddenforest.co.nz/index.htm

http://www.effnz.co.nz/

http://www.nzbrn.org.nz/

http://www.ngamanu.co.nz/

Species List

Legend

*          Exotic

?          Identification uncertain

AS       Akatarawa Saddle

CW      Cambourne Walkway Porirua

ER       El Rancho Camp

HM      Hemi Matenga Scenic Reserve

LP       Lake Papaitonga

LR       Lake Reserve, Waikanae Rivermouth

MH      Mount Holdsworth

NM      Nga Manu Nature Reserve

MW     Mangaone Walkway

NR      Nikau Reserve

OF       Otaki Forks

OV      Orongorongo Valley

PP        Peka Peka Beach

PSR     Paraparaumu Scenic Reserve

RFP     Rimutaka Forest Park

WB      Waikanae Beach

WR      Waikawa Stream Reserve

WSR   Waiopehu Scenic Reserve

WT      Waiotauru Track